Building limb buds

Building limb buds

This workshop on limb development was a highly enjoyable meeting that covered a wide range of topics related to appendage development and evolution. The discussions were vivid, open and stimulating for all participants. It was particularly interesting to see how broadly accepted concepts, such as the morphogen models in the fly and the progress zone model in vertebrates, were re-evaluated on th...

Effect of Thyroxine on Early Development of Chick Limb Buds

Experiments on developing limb buds of the axolotl Ambystoma mexicanum.

Various experiments were performed on the limb buds of axolotls to compare the behaviour of amphibian limbs with that previously reported for chick limbs. Following removal of the tip or whole limb bud, extensive powers of regulation were observed since complete limbs always formed. Similarly after distal to proximal grafts intercalary regulation occurred to produce perfect limbs and after prox...

Morphogenetic rearrangement of injected collagen in developing chicken limb buds.

A fundamental question concerning the development of the extracellular matrix is what factors control the arrangement of collagen fibrils within a tissue and at the same time allow for the great diversity of geometric forms exhibited by collagen. In this report, we test the possibility that physical forces within the embryo serve to organize collagen fibers into regular patterns. In particular,...

Congenic method in the chick limb buds by electroporation.

Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protoc...